Journal: Cell reports

Article Title: Activation of GPR44 decreases severity of myeloid leukemia via specific targeting of leukemia initiating stem cells

doi: 10.1016/j.celrep.2023.112794

Figure Lengend Snippet:

Article Snippet: Mouse monoclonal anti-Raf-1 (E-10) , Santa Cruz Biotechnology , Cat# sc-7267; RRID: AB_628196.

Techniques: Virus, Recombinant, Enzyme-linked Immunosorbent Assay, Binding Assay, Staining, Modification, Saline, Concentration Assay, Over Expression, Protein Extraction, Membrane, SYBR Green Assay, Bicinchoninic Acid Protein Assay, Protease Inhibitor, CCK-8 Assay, Reverse Transcription, Selection, Plasmid Preparation, Knock-Out, Software, Real-time Polymerase Chain Reaction, Flow Cytometry

Journal: Heliyon

Article Title: Multi-omics reveals aging-related pathway in natural aging mouse liver

doi: 10.1016/j.heliyon.2023.e21011

Figure Lengend Snippet: Changes of proteins in the MAPK pathway. (A) Western blot (WB) detected the expression levels of BRAF, MEK, and ERK. The original WB images were available in the supplementary file. (B, C) BRAF protein expression in young and old mice. (D, E) MEK protein expression in young and old mice. (F, G) ERK protein expression in young and old mice. (***) P < 0.05.

Article Snippet: The primary antibodies against BRAF (proteintech), MEK (Abcam), ERK (proteintech), GAPDH (proteintech), and the mouse IgG (H + L) secondary antibody (Pierce, Rockford, USA), rabbit IgG (H + L) secondary antibody (Pierce, Rockford, USA) were used.

Techniques: Western Blot, Expressing